Tratamento de lesão periradicular, de origem endodôntica, com auxílio de laserterapia: uma revisão sistemática da literatura e apresentação se caso clínico / Treatment of periradicular injury, of endodontic origin, with the aid of laser therapy: a systematic review of the literature and presentation if clinical case

É sabido que a cavidade oral é a porta de entrada para muitos microrganismos e que possui alguns gêneros de bactérias que quando se associam ou proliferam em excesso, com a falta de higienização correta, fatores ambientais e imunológicos podem causar danos em algumas partes dos dentes como no tecido pulpar e canais radiculares. Neste sentido, a endodontia tem sido capaz de tratar pessoas com problemas dentários em fases muito precoces como as medidas profiláticas até casos infeciosos que afetam a polpa dos dentes causando as lesões perirradiculares que são infecções causadas por microrganismos, Para tanto, é necessário que a odontologia ofereça transformações positivas por meio de técnicas que sejam mais previsíveis para os tratamentos. A literatura tem indicado o uso da laserterapia como técnica auxiliar para o tratamento endodôntico pelo seu potencial mínimo invasivo, preciso e eficaz em menor tempo de cura de patologias orais como as lesões perirradiculares. Este estudo pretendeu abordar a importância da laserterapia sobre as variáveis patológicas de pacientes com lesão perirradiculares. Para realiza-lo foi necessário buscar na literatura estudos que associem este tipo de tratamento e sua eficiência no tratamento desta patologia. Para realizar esta pesquisa foi necessário buscar dados na literatura que tratam sobre o tema para investigar o tempo indicado ao tratamento com a laserterapia bem como o estágio em que as lesões podem ser tratadas com auxílio deste método. Corroborando com o estudo apresentamos um caso clínico(AU)

It is known that the oral cavity is the gateway to many microorganisms and that it has some genera of bacteria that when they associate or proliferate in excess, with the lack of correct hygiene, environmental and immunological factors can cause damage in some parts of the teeth such as in pulp tissue and root canals. In this sense, endodontics has been able to treat people with dental problems at very early stages such as prophylactic measures to infectious cases that affect the pulp of teeth causing perirradicular lesions that are infections caused by microorganisms, therefore, it is necessary that dentistry offer positive transformations through techniques that are more predictable for treatments. The literature has indicated the use of laser therapy as an auxiliary technique for endodontic treatment for its minimal invasive, precise and effective potential in shorter time of cure of oral pathologies such as perirradicular lesions. This study aimed to address the importance of laser therapy on the pathological variables of patients with perirradicular lesions. To accomplish this, it was necessary to seek studies in the literature that associate this type of treatment and its efficiency in the treatment of this pathology. To carry out this research it was necessary to seek data in the literature that deal with the subject to investigate the time indicated for treatment with laser therapy as well as the stage at which lesions can be treated with the aid of this method. Corroborating the study, we present a clinical case(AU)

Vigilancia clínica-microbiológica de una Periodontitis Grado C etapa III en cinco años / Clinical-microbiological vigilance of a C Grade Periodontitis Stage III in five years

Resumen Introducción: Diversas técnicas se utilizan para tratar y mantener los dientes afectados con Periodontitis de estadio III grado C, sin embargo, hay poca información sobre cómo se modifican los parámetros clínicos periodontales y la composición microbiológica durante el tratamiento convencional y quirúrgico. Objetivo: Evaluar la respuesta clínica y microbiológica en una paciente con periodontitis estadio III grado C generalizada durante 5 años. Cuyo tratamiento consistió en terapia periodontal no quirúrgica y quirúrgica utilizando material regenerativo y sustituto óseo. Metodología: Se realizó raspado y alisado radicular progresivo, combinado con antibióticos y cirugía en sitios con defectos infraóseos. Se tomó registro de placa bacteriana subgingival (PB), hemorragia (H), profundidad de la bolsa (PS) y nivel de inserción clínica (NIC) en cada momento del tratamiento. Se tomaron muestras de la profundidad de las bolsas para identificar bacterias periodontales por biología molecular. Resultados: Se observó una mejoría de PB, H, PS y ganancia de NIC a lo largo de los 5 años. Con los injertos óseos la PS disminuyó 5 mm y de NIC se ganó 5 mm, con amelogeninas las diferencias fueron de 4,5 mm respectivamente. En colgajos de acceso, la PS disminuyó 3 mm y de NIC se ganó 2 mm. Se identificó T.denticola a los 36 meses, en todas las bolsas tratadas con colgajo de acceso y en el 50% de las bolsas con injertos óseos; y P. gingivalis a los 60 meses. Conclusiones: El tratamiento periodontal aplicado evitó la pérdida de los dientes afectados. El mejoramiento de los parámetros clínicos se asoció con una microbiota no agresiva.

Abstract Introduction: There are several techniques to treat and maintain teeth affected by stage III, grade C periodontitis, nonetheless, the scientific evidence available on how periodontal clinical parameters and microbiological composition may be modified during the conventional and surgical treatment is scarce. Objective: To evaluate the clinical and microbiological response of a patient with stage III grade C, generalized periodontitis, during 5 years, treated with non-surgical and surgical periodontal therapy using regenerative material and bone substitute. Methodology: The patient was treated with scaling and progressive root planning, combined with antibiotics and surgical therapy was performed in sites with infraosseous defects. At each time of treatment, subgingival bacterial plaque (PB), haemorrhage (H), probing depth (PD) and clinical attachment level (CAL) were recorded. To identify periodontal bacteria by molecular biology samples were taken with endodontic cones from the pocket depth. Results: A significant difference of PB, H, PD was observed. The PD decreased and CAL was gained throughout the treatment. PD decreased 5 mm with the application of bone substitute, and CAL gained 5 mm, with the use of amelogenins the difference of PD and CAL was 4.5 mm. In access flap the PD decreased 3 mm and the CAL improved 2 mm. T. denticola was identified at 36 months in all pockets treated with access flap and in 50% of the pockets with bone graft, and P. gingivalis at 60 months. Conclusions: The periodontal treatment applied prevented the loss of the affected teeth. Improvement of clinical parameters was associated with a non-aggressive microbiota.

Practice and thoughts of integrating curriculum ideological and political education into oral microbiology course / 中华医学教育探索杂志

This study explores the process of introducing ideological and political education elements into oral microbiology teaching, and helps students to establish Marxist practical, epistemological and scientific and technological views, understand the strategic connotation of healthy China in the new era, and establish a sense of responsibility to serve the needs of the country and promote social development. Finally, through the collection of students' feedback, the implementation effect is summarized and the existing problems have been improved in a targeted way.

Análise da eficácia da higiene oral de pacientes internados em unidade de terapia intensiva em um hospital de alta complexidade do sul do Brasil / Analysis of the effectiveness of oral hygiene of interns patients in intensive therapy unit in a high-complexity hospital in the south of Brazil

Introdução: As infecções orais, na Unidade de Terapia Intensiva (UTI), deveriam ser preocupações constantes dos profissionais da área da Saúde ali inseridos, devido as consequências que podem causar na saúde geral dos pacientes debilitados sistemicamente. A criação de um protocolo padrão de higiene oral e de suma importância para impedir ou tratar tais infecções, o que possibilita ao paciente conforto e qualidade de vida, devendo ser realizada por profissionais qualificados. Métodos: Foi realizado um estudo transversal e descritivo, cuja analise foi descritiva e se desenvolveu na Unidade de Terapia Intensiva de um hospital de alta complexidade no Sul do Brasil, no período de fevereiro de 2016 a fevereiro de 2017. A amostra total foi composta por 35 pacientes, com idade mínima de 18 anos, que estavam internados na UTI do referido hospital, portadores de prontuários e Termo de Consentimento Livre e Esclarecido. Resultados: Dentre os microrganismos achados nos exames laboratoriais dos pacientes, apresentaram-se em maior quantidade Klebsiella pneumoniae, Staphylococcus coagulase negativo e Escherichia coli. Apenas dois pacientes adquiriram o Acinetobacter baumannii. A maioria dos pacientes obtiveram bactérias gram-negativas presentes em sua microbiota oral. Conclusões: As bactérias patogênicas presentes no meio oral devem ser tratadas e erradicadas. Isso pode ser alcançado por meio de um protocolo padrão de higiene oral. A participação da Odontologia na equipe multidisciplinar no ambiente hospitalar e de fundamental importância para a indicação da terapêutica adequada.

Introduction: Oral infections in the Intensive Care Unit (ICU) should be a constant concern of the health professionals inserted there, due to the consequences that can cause in the general health of systemically debilitated patients. The creation of a standard protocol of oral hygiene is of paramount importance to prevent or treat such infections, allowing the patient comfort and quality of life and should be performed by qualified professionals. Methods: A cross-sectional and descriptive study was carried out, which was descriptive and developed in the Intensive Care Unit of a highly complex hospital in the South of Brazil, from February 2016 to February 2017. The total sample was composed by 35 patients with a minimum age of 18 years who were hospitalized in the ICU of the referred hospital, patients with medical records and a Consent Form, free and clear. Results: Among the microorganisms found in the laboratory exams of the patients, Klebsiella pneumoniae, Staphylococcus coagulase negative and Escherichia coli were present.Only two patients acquired Acinetobacter baumannii. Most of the patients obtained gram negative bacteria present in their oral microbiota. Conclusions: The pathogenic bacteria that are present in the oral environment must be treated and eradicated. This can be achieved through a standard oral hygiene protocol. The participation of Dentistry in the multidisciplinary team in the hospital environment is of fundamental importance for the indication of the appropriate therapy

Microbiota das infecções endo-periodontais: uma revisão de literatura / Microbiota of endo-periodontal infections: a literature review

O objetivo desse estudo é revisar a literatura acerca da microbiologia presente nas doenças endoperiodontais e assim apresentar um melhor conhecimento ao clínico frente à patologia a ser tratada. Foi realizada uma busca nas plataformas: Google Acadêmico, Pubmed e Scielo. Foram selecionados artigos de 2007 até 08/2018, em língua inglesa e língua portuguesa, com os termos: "Endodontia" E/OU "Lesão endoperiodontal E/OU "Microbiologia oral" E/OU "Periodontia". Os principais resultados encontrados demonstraram uma grande variedade microbiológica associada às lesões endoperiodontais e ainda, a presença de alguns microrganismos de difícil eliminação. Na lesão endodôntica primária: Enterococcus faecalis, Parvinonas micra, Mogibacterium timiduam, Filifactor alocis, Fretibacterium fastidiosum, Parvinonas micra, Streptococcus constellatus, Eubacterium brachy, Tannerella forsythia. Anaeróbios estritos: Veillonella parvula, Porphyromonas gingivalis, Propionibacterium acnes, Lactobacillus acidophilus, Campylobacter rectus, Slackia exigua, Anaeróbios facultativos: Bactérias microaerofílicas: Aggregatibacter actinomycetemcomitans. Lesão endodôntica primária com envolvimento periodontal secundário: Enterococcus faecalis, Parvinonas micra, Mogibacterium timiduam, Filifactor alocis, Fretibacterium fastidiosum, Streptococcus constellatus, Eubacterium brachy, Tannerella forsythia; Lesão periodontal primária: Porphyromonas gingivalis, Fusobacterium nucleatum, Eikenella corrodens Fusobacterium nucleatum, Treponema denticola, Tannerella forsythia, Prevotella intermedia, Parvimonas micra. Lesão periodontal primária com envolvimento endodôntico secundário: Bacteroidaceae sp., Fretibacterium fastidiosum. Lesão endoperiodontal verdadeira combinada: Tannerella forsythia, Porphyromonas endodontalis, Aggregatibacter aphrophilus, Peptostreptococcus stomatitis, Veillonella rogosae, Campylobacter rectus, Campylobacter concisus, Neisseria elongata; Veillonella rogosae, Fusobacterium canifelinum, Haemophilus parainfluenzae, Peptostreptococcus stomatitis, Peptostreptococcus stomatitis, Enterobacter asburiae, Aggregatibacter aphrophilus, Campylobacter rectus, Corynebacterium matruchotii, Neisseria bacilliformis, Actinomyces odontolyticus, Mogibacterium timidum. Conclui-se que o cirurgião-dentista deve melhor identificar qual a microbiota presente em cada tipo de patologia endoperiodontal para que consiga realizar os tratamentos com eficiência obtendo o sucesso(AU)

The objective of this study is to review the literature about the microbiology present in endodontic diseases and thus to present a better knowledge of the clinician regarding the pathology to be treated. By searching the platforms: Google Scholar, Pubmed and Scielo. Articles from 2007 to 08/2018, in English and Portuguese, were selected using the terms: "Endodontics" AND/OR "Endo-periodontal lesion AND/OR" Oral Microbiology "AND/OR" Periodontics ". The main results of the search were a large microbiological variety associated with endo-periodontal lesions and the presence of some microorganisms that were difficult to eliminate. Primary endodontic lesion: Enterococcus faecalis, Parvinonas micra, Mogibacterium timiduam, Filifactor alocis, Fretibacterium fastidiosum, Parvinonas micra, Streptococcus constellatus, Eubacterium brachy, Tannerella forsythia. The primary endodontic lesions with secondary periodontal involvement are: Enterococcus faecalis, Parvinonas micra, Mogibacterium timiduam, Filifactor alocis, Fretibacterium, Agrigatibacter actinomycetemcomitans, Staphylococcus aureus, Campylobacter rectus, Campylobacter rectus, Slackia exigua fastidiosum, Streptococcus constellatus, Eubacterium brachy, Tannerella forsythia. Primary periodontal lesion: Porphyromonas gingivalis, Fusobacterium nucleatum, Eikenella corrodens Fusobacterium nucleatum, Treponema denticola, Tannerella forsythia, Prevotella intermedia, Parvimonas micra; Primary periodontal lesion with secondary endodontic involvement: Bacteroidaceae sp., Fretibacterium fastidiosum. True endo-periodontal lesion combined: Tannerella forsythia, Porphyromonas endodontalis, Aggregatibacter aphrophilus, Peptostreptococcus stomatitis, Veillonella rogosae, Campylobacter rectus, Campylobacter concisus, Neisseria elongata;Veillonella rogosae, Fusobacterium canifelinum, Haemophilus parainfluenzae, Peptostreptococcus stomatitis, Peptostreptococcus stomatitis, Enterobacter asburiae, Aggregatibacter aphrophilus, Campylobacter rectus, Corynebacterium matruchotii, Neisseria bacilliformis, Actinomyces odontolyticus, Mogibacterium timidum.It concludes that the dental surgeon must identify which microbiota is present in each type of endo-periodontal pathology, so that he can perform the treatments efficiently, achieving success. (AU)

Vigilancia clínica-microbiológica de una Periodontitis Grado C etapa III en cinco años

Introducción: Diversas técnicas se utilizan para tratar y mantener los dientes afectados con Periodontitis de estadio III grado C, sin embargo, hay poca información sobre cómo se modifican los parámetros clínicos periodontales y la composición microbiológica durante el tratamiento convencional y quirúrgico. Objetivo: Evaluar la respuesta clínica y microbiológica en una paciente con periodontitis estadio III grado C generalizada durante 5 años. Cuyo tratamiento consistió en terapia periodontal no quirúrgica y quirúrgica utilizando material regenerativo y sustituto óseo. Metodología: Se realizó raspado y alisado radicular progresivo, combinado con antibióticos y cirugía en sitios con defectos infraóseos. Se tomó registro de placa bacteriana subgingival (PB), hemorragia (H), profundidad de la bolsa (PS) y nivel de inserción clínica (NIC) en cada momento del tratamiento. Se tomaron muestras de la profundidad de las bolsas para identificar bacterias periodontales por biología molecular. Resultados: Se observó una mejoría de PB, H, PS y ganancia de NIC a lo largo de los 5 años. Con los injertos óseos la PS disminuyó 5 mm y de NIC se ganó 5 mm, con amelogeninas las diferencias fueron de 4,5 mm respectivamente. En colgajos de acceso, la PS disminuyó 3 mm y de NIC se ganó 2 mm. Se identificó T.denticola a los 36 meses, en todas las bolsas tratadas con colgajo de acceso y en el 50% de las bolsas con injertos óseos; y P. gingivalis a los 60 meses. Conclusiones: El tratamiento periodontal aplicado evitó la pérdida de los dientes afectados. El mejoramiento de los parámetros clínicos se asoció con una microbiota no agresiva.

Introduction: There are several techniques to treat and maintain teeth affected by stage III, grade C periodontitis, nonetheless, the scientific evidence available on how periodontal clinical parameters and microbiological composition may be modified during the conventional and surgical treatment is scarce. Objective: To evaluate the clinical and microbiological response of a patient with stage III grade C, generalized periodontitis, during 5 years, treated with non-surgical and surgical periodontal therapy using regenerative material and bone substitute. Methodology: The patient was treated with scaling and progressive root planning, combined with antibiotics and surgical therapy was performed in sites with infraosseous defects. At each time of treatment, subgingival bacterial plaque (PB), haemorrhage (H), probing depth (PD) and clinical attachment level (CAL) were recorded. To identify periodontal bacteria by molecular biology samples were taken with endodontic cones from the pocket depth. Results: A significant difference of PB, H, PD was observed. The PD decreased and CAL was gained throughout the treatment. PD decreased 5 mm with the application of bone substitute, and CAL gained 5 mm, with the use of amelogenins the difference of PD and CAL was 4.5 mm. In access flap the PD decreased 3 mm and the CAL improved 2 mm. T. denticola was identified at 36 months in all pockets treated with access flap and in 50% of the pockets with bone graft, and P. gingivalis at 60 months. Conclusions: The periodontal treatment applied prevented the loss of the affected teeth. Improvement of clinical parameters was associated with a non-aggressive microbiota.

Avaliação da atividade antibacteriana do hidróxido de cálcio associado a diferentes veículos em blocos de dentina contaminados por Enterococcus faecalis / Evaluation of the antibacterial activity of calcium hydroxide associated different vehicles in dentin blocks contaminated with Enterococcus faecalis

Introdução: A infecção endodôntica ocasionada por Enterococcus faecalis é um problema sério no tratamento de dentes comprometidos. É de preocupação do cirurgião dentista um completo saneamento do sistema de canais radiculares pela aplicação de medicação antisséptica entre sessões. Dentre estas medicações, a pasta de hidróxido de cálcio tem sido associada a diferentes veículos para potencializar sua ação. Objetivos: Diante disto, este estudo objetivou avaliar e comparar a eficiência da atividade antimicrobiana in vitro de diferentes pastas de hidróxido de cálcio frente ao E. faecalis. Material e Métodos: Os testes foram executados em 49 blocos de dentina infectados com E. faecalis e tratados com pastas de hidróxido de cálcio em diferentes veículos por uma semana. A eficiência das pastas foi avaliada pela microscopia confocal de varredura a laser. Para comparação entre as pastas foi empregado o teste de Kruskal-Wallis e pelo teste de Dunn para comparações individuais com nível de significância estabelecido em 5%. Resultados: A aplicação das diferentes pastas proporcionou uma significativa alteração na proporção de bactérias viáveis e não viáveis encontradas no biovolume celular total dos blocos de dentina. Conclusão: A pasta que revelou melhor desempenho antimicrobiano foi aquela cujo veículo foi água destilada. A pasta de hidróxido de cálcio associada ao extrato propilenoglicólico de guaçatonga não apresentou desempenho antimicrobiano sobre células de E. faecalis. (AU)

Introduction: The endodontic infection caused by Enterococcus faecalis is a serious problem in the treatment of compromised teeth. It is a concern to the dental surgeon a complete sanitation of the root canal system by applying antiseptic medication between sessions. Among these medications, the calcium hydroxide paste has been linked to different vehicles to enhance its action. Objectives: Thus, this study aimed to evaluate and compare the efficiency of in vitro antimicrobial activity of different pastes of calcium hydroxide against E. faecalis. Material and Methods: Tests were performed in 49 blocks dentin infected with E. faecalis and treated with calcium hydroxide pastes in different vehicles for a week. The efficiency of the pastes was evaluated by confocal laser scanning. The Kruskal-Wallis test was used to compare the pastes and the Dunn test was used for individual comparisons with a significance level set at 5%. Results: The application of different pastes provided a significan change in the proportion of viable and non-viable bacteria found in the total cell biovolume of the blocks of dentin. Conclusion: The paste that revealed the best antimicrobial performance was the one whose vehicle was distilled water. The calcium hydroxide paste associated with the extract of guaçatonga in propylene glycol showed no antimicrobial performance on cells of E. faecalis. (AU)

Halistanol sulfate A and rodriguesines A and B are antimicrobial and antibiofilm agents against the cariogenic bacterium Streptococcus mutans

In the present investigation we report the antibacterial activity of halistanol sulfate A isolated from the sponge Petromica ciocalyptoides, as well as of rodriguesines A and B isolated from the ascidian Didemnum sp., against the caries etiologic agent Streptococcus mutans. The transcription levels of S. mutans virulence genes gtfB, gtfC and gbpB, as well as of housekeeping genes groEL and 16S, were evaluated by sqRT-PCR analysis of S. mutans planktonic cells. There were no alterations in the expression levels of groEL and 16S after antimicrobial treatment with halistanol sulfate A and with rodriguesines A and B, but the expression of the genes gtfB, gtfC and gbpB was down-regulated. Halistanol sulfate A displayed the most potent antimicrobial effect against S. mutans, with inhibition of biofilm formation and reduction of biofilm-associated gene expression in planktonic cells. Halistanol sulfate A also inhibited the initial oral bacteria colonizers, such as Streptococcus sanguinis, but at much higher concentrations. The results obtained indicate that halistanol sulfate A may be considered a potential scaffold for drug development in Streptococcus mutans antibiofilm therapy, the main etiologic agent of human dental caries. .

Implementing microbiology course reform for stomatology majors / 中华医学教育探索杂志

This article introduced the thoughts and practice of implementing microbiology course reform for stomatology majors in school of stomatology and department of microbiology in Tianjin medical university.To make the introductory courses more suitable and meet the needs of stomatology better,our microbiology classes included the basic knowledge of oral microoganism.

PCR e o perfil microbiológico das infecções endodônticas: realidade ou utopia? / PCR and the microbiological profile of endodontic infections: reality or utopy?

A influência dos microorgaismos nos processos patológicos, principalmente, infecciosos que envolvem a polpa e o periápice, está bem estabelecida nas falhas dos tratamentos endodônticos; entretanto, o real papel que cada microorganismo exerce dentro do processo infeccioso ainda é capaz de levantar discussões. Dentro desse contexto, o uso de novas técnicas moleculares, mais sensíveis e de maior especificidade na identificação desses patógenos abre novas perspectivas no campo da pesquisa, diagnóstico, tratamento e desenvolvimento de novos materiais endodônticos. O objetivo desse trabalho é o de discutir, à luz da literatura, as reais abrangências e possibilidades de uma dessas técnicas - a PCR (poliymerase chain reaction) - dentro do âmbito do conhecimento e identificação dos patógenos responsáveis pelas infecções endodônticas.

The influence of microorganisms in pathological processes is well established in the flaws of endodontic treatment, especially infectious processes involving the pulp and periapex. However, the actual role that each microorganism exercises within the infectious process remains open for discussion. Thus, the use of new molecular methods with greater sensitivity and specificity in the identification of these pathogens broadens perspectives in the s of research, diagnosis, treatment and the development od new endodontic materials. The aim of the present study was to discuss the scope and possibilities of one such method - PCR (polymerase chain reaction) - regarding knowledge and the identification of pathogens that cause endodontic infection.